رحاب صبحي رمضان
  • EARLY DETECTION OF PROLACTIN HORMONE ELEVATION IN WOMEN SUFFERING FROM INFERTILITY
  • This work was constructed to discuss a sensitive issue regarding infertility cases that was attributed to 
    hyperprolactinemia. About 150 blood samples were collected from females suffering hyperprolactinemia and 50 from normal
    subjects served as control for comparison. Test subjects were divided into three age groups: 20-30 years old, 31-40 years old
    and 41-50 years old. Tests of fertility hormones luteinizing hormone, follicle stimulation hormone and prolactin were
    performed for all subjects under study.  It was found that there is a significant P<0.05 difference in hormone concentration for
    patients when compared to normal. LH and FSH recorded significant decrease while prolactin recorded a significant increase
    when compared to normal. RNA isolation from serum was possible due to high expression of prolactin gene in patients since an
    average concentration of 200 ng was obtained from serum. At molecular level analysis using three specific primers designed for
    this study, showed that there is an aberration at expression level of RNA in some of hyperprolactinemia patients while prolactin
    receptors were normal in patients studied. It was concluded that in all patients feedback inhibition mechanism that controls
    prolactin level, was disrupted in addition some of patients studied were candidates for breast cancer as was reviewed from
    family history and most of hyperprolactinemia cases for subjects studied were attributed to hyper expression of prolactin gene.
    Protein analysis of blood from patients showed that a significant increase in albumin, this was regarded to increased level of
    prolactin in blood and this protein functions as a carrier for hormones.