ا.م.د. رحاب صبحي رمضان
  • DEVELOPMENT OF REAL TIME PCR ASSAY FOR EARLY DETECTION OF CYTOMEGALOVIRUS INFECTION IN IRAQI WOMEN
  • This study was constructed to discuss a Molecular Study of
    Cytomegalovirus isolated from women with repeated miscarriage in
    relation to immune response molecule Tall like Receptor2. About
    (100) blood samples from women suffering from infection with
    Cytomegalovirus were collected from infertility clinic of Kamal Al –
    Sammaraee hospital and (50) samples from normal subjects served as
    control for comparison. Test subjects were divided into two age groups: 20-30 years old and 31-40 years old. The women distributed as (60) samples of
    infertile and (40) samples as miscarriage women. This study included Enzyme Linked
    Immune Sorbent Assay (ELISA) test was used to detect anti- HCMV antibodies IgG and IgM
    in the patient serum samples. ELISA test result showed that the miscarriage women shown
    highest percentage of seropositive to CMV for IgG (40%) and (25%) IgM compared to
    infertile women IgG (20%) and (15%) IgM with a significant difference P<0.05.  It has been
    concluded that there is high prevalence of anti- HCMV antibodies IgG and IgM in both
    miscarriage and infertile women compared with normal (healthy) women in Baghdad, The
    Seropositive of anti- HCMV IgG was higher in younger women (20-30) years old while the
    largest age classes (31-40) years showed higher level of IgM. The CMV primers were 
    selected from highly conserved region of the major enveloped glycoprotein B (gB) and used 
    probe labeled at the 5 end with FAM and the 3 end with TAMRA. The result shown
    amplification from the sixth cycle.