This study was constructed to discuss a Molecular Study of
Cytomegalovirus isolated from women with repeated miscarriage in
relation to immune response molecule Tall like Receptor2. About
(100) blood samples from women suffering from infection with
Cytomegalovirus were collected from infertility clinic of Kamal Al –
Sammaraee hospital and (50) samples from normal subjects served as
control for comparison. Test subjects were divided into two age groups: 20-30 years old and 31-40 years old. The women distributed as (60) samples of
infertile and (40) samples as miscarriage women. This study included Enzyme Linked
Immune Sorbent Assay (ELISA) test was used to detect anti- HCMV antibodies IgG and IgM
in the patient serum samples. ELISA test result showed that the miscarriage women shown
highest percentage of seropositive to CMV for IgG (40%) and (25%) IgM compared to
infertile women IgG (20%) and (15%) IgM with a significant difference P<0.05.  It has been
concluded that there is high prevalence of anti- HCMV antibodies IgG and IgM in both
miscarriage and infertile women compared with normal (healthy) women in Baghdad, The
Seropositive of anti- HCMV IgG was higher in younger women (20-30) years old while the
largest age classes (31-40) years showed higher level of IgM. The CMV primers were 
selected from highly conserved region of the major enveloped glycoprotein B (gB) and used 
probe labeled at the 5 end with FAM and the 3 end with TAMRA. The result shown
amplification from the sixth cycle.