رحاب صبحي رمضان
  • Molecular analysis of prolactin gene exons region of hyperprolactinemic and breast cancer Iraqi patients
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    This study was constructed to investigate hyperprolactinemia related
    infertility through the molecular base   associated with single nucleotide
    polymorphism (SNP) at prolactin gene  in hyperprolactemic patients with the
    breast cancer risk. This study included one hundred fifty  blood samples from
    patients suffering  hyperprolactinemia and infertility. Also twenty five tissue
    samples of breast cancer patients were collected in which fifteen samples
    were frozen tissue and ten samples were formalin fixed paraffin embedded
    tissue. Fifty blood samples from healthy persons were collected served as
    control group. The main ages of patients were 20 to 50 and same for control
    (healthy) group. it’s clear that  there is   substitution and  deletion mutation, 
    in which the highest mutation number was in exon 2, which was 9 mutations,
    all mutations in this exon was substitution ,while the less mutation number
    was in exon 3 and exon4 which was 2 for each exon, one substitution and
    one deletion mutation in exon 3 while the two mutations in exon 4 was
    substitution  only. The risk association between the DNA  of
    hyperprolactemic and breast cancer patients using information on national
    center for biotechnology information (NCBI), and Mega 6  program.