This study was constructed to investigate hyperprolactinemia related
infertility through the molecular base   associated with single nucleotide
polymorphism (SNP) at prolactin gene  in hyperprolactemic patients with the
breast cancer risk. This study included one hundred fifty  blood samples from
patients suffering  hyperprolactinemia and infertility. Also twenty five tissue
samples of breast cancer patients were collected in which fifteen samples
were frozen tissue and ten samples were formalin fixed paraffin embedded
tissue. Fifty blood samples from healthy persons were collected served as
control group. The main ages of patients were 20 to 50 and same for control
(healthy) group. it’s clear that  there is   substitution and  deletion mutation, 
in which the highest mutation number was in exon 2, which was 9 mutations,
all mutations in this exon was substitution ,while the less mutation number
was in exon 3 and exon4 which was 2 for each exon, one substitution and
one deletion mutation in exon 3 while the two mutations in exon 4 was
substitution  only. The risk association between the DNA  of
hyperprolactemic and breast cancer patients using information on national
center for biotechnology information (NCBI), and Mega 6  program.