ا.م.د. رحاب صبحي رمضان
  • Genetic risk factors associated with thrombosis in a sample of Iraq population
  • This study aimed to investigate the association of F5 gene Single Nucleotide Polymorphism
    (SNP) with the incidence of thrombosis. Blood samples were collected from 40 patients during
    the period from November 2014 to January 2015, from Critical Care Unit (CCU) of (Yarmook
    Hospital, Kadhimiya Hospital), as well as from 10 unrelated healthy control group. This study
    found the age group between 50 to 60 are more susceptible to thrombosis 45% and the thrombosis
    was more frequent in male 55% from female 45% the significant (p<0.01). Deoxyribonucleic
    acid (DNA) was extracted from whole blood samples, whereas, serum samples were analyzed
    using troponin test (TNT) for detection of thrombosis. Polymerase Chain Reaction (PCR) was
    achieved on extracted DNA using eleven specific primers for F5 gene :the first primer (Fve3)
    with product size (228bp), second primer (Fve4) with product size (310bp), third primer (Fve6)
    with product size(547bp), fourth primer (Fve7) with product size (241bp), fifth primer (Fve8)
    with product size (306bp), sixth primer (Fve12) with product size (286bp), seventh primer
    (Fve13a) with product length (260bp), eighth primer (Fve13c) with product size (317bp), ninth
    primer (Fve15) with product size (600bp), tenth primer (Fve16) with product size (333bp) and
    eleventh primer (Fve25) with product size (390bp). PCR products of F5 gene were sequenced.
    Result found to be change in DNA which was mostly SNP. This change was in three types:
    substitution 24.86%, insertion 28.57% and deletion 28.57%, a Leiden mutation was also identified
    among patients.