رحاب صبحي رمضان
  • PHYSIOLOGICAL AND MOLECULAR STUDY ON POLYCYSTIC OVARY SYNDROME IN A SAMPLE OF PHYSIOLOGICAL AND MOLECULAR STUDY ON POLYCYSTIC OVARY SYNDROME IN A SAMPLE OF IRAQI WOMEN
  • This study investigates Poly Cystic Ovary Syndrome (PCOS) relation to infertility through a
    biochemical and molecular at methylene tetrahydrofolate reductase (MTHFR) gene and PCOS gene.
    Samples from patients suffering PCOS were collected from Kamal al
    tissue samples were collected from Madinat al
    samples from healthy women served as the control. Average ages of pa
    20-50 years. Subjects were divided in to three age groups (20
    Serum samples for all PCOS patients were measured for fertility hormones levels like Luteinizing
    Hormone (LH), Follicle Stimula
    recorded a significant decreased in age group (41
    significant increase in age group (31
    detected in PCOS gene. Polymerase chain reaction (PCR) was done using a specific primers set to
    amplify exon (2) of the methylene tetrahydrofolate reductase (MTHFR) gene. Another three primers
    were designed to amplify the exons (5
    sequencing that the percentage of substitution mutation was 88%, while the deletion mutation percent
    was 12%. 
    This study investigates Poly Cystic Ovary Syndrome (PCOS) relation to infertility through a
    biochemical and molecular at methylene tetrahydrofolate reductase (MTHFR) gene and PCOS gene.
    Samples from patients suffering PCOS were collected from Kamal al
    tissue samples were collected from Madinat al-Amamin Al-Kazimin Al
    samples from healthy women served as the control. Average ages of pa
    50 years. Subjects were divided in to three age groups (20-30), (31
    Serum samples for all PCOS patients were measured for fertility hormones levels like Luteinizing
    Hormone (LH), Follicle Stimulating Hormone FSH and Testosterone hormone.
    recorded a significant decreased in age group (41-50) years old, while LH and Testosterone recorded a
    significant increase in age group (31-40) years old. The study confirmed the incidence of SNPs
    detected in PCOS gene. Polymerase chain reaction (PCR) was done using a specific primers set to
    amplify exon (2) of the methylene tetrahydrofolate reductase (MTHFR) gene. Another three primers
    were designed to amplify the exons (5-10) regions of (PCOS) gen
    sequencing that the percentage of substitution mutation was 88%, while the deletion mutation percent
    was 12%.  
    This study investigates Poly Cystic Ovary Syndrome (PCOS) relation to infertility through a
    biochemical and molecular at methylene tetrahydrofolate reductase (MTHFR) gene and PCOS gene.
    Samples from patients suffering PCOS were collected from Kamal al-Samarrai Hospital. Twenty
    Kazimin Al-Tibbia Hospital. Fifty blood
    samples from healthy women served as the control. Average ages of patients and control group were
    30), (31-40) and (41-50), years old.
    Serum samples for all PCOS patients were measured for fertility hormones levels like Luteinizing
    ting Hormone FSH and Testosterone hormone. The FSH hormone
    50) years old, while LH and Testosterone recorded a
    40) years old. The study confirmed the incidence of SNPs
    detected in PCOS gene. Polymerase chain reaction (PCR) was done using a specific primers set to
    amplify exon (2) of the methylene tetrahydrofolate reductase (MTHFR) gene. Another three primers
    10) regions of (PCOS) gene. It was found after PCR product
    sequencing that the percentage of substitution mutation was 88%, while the deletion mutation percent