رحاب صبحي رمضان
  • Molecular Diagnosis on Glucose 6 Phosphate Dehydrogenase in a Sample of Iraqi Patients
  • This study was constructed to invertigate  Glucose 6 phosphate dehydrogenase
    deficiency, and the genetic disorder that leading to heamolysis anemia in group of Iraqi
    patient. A total of 50 blood samples were collected from different hospitals (Yarmook
    Hospital, Child hospital , AL Alweyaa , and Medical City) these samples varied in severity
    in of the enzyme deficiency from mild to chronic according to the test of G6PD, and 20
    samples served as control (healthy). The period time of samples collection took about
    three months from March to June 2014. The percentage of mild symptoms was 42% , acute
    symptoms was 40% and carrier G6PD was 18% as stated on statistics analysis of Chi
    square. DNA was extracted from samples and subjected to PCR amplification using 3
    specific primers designed for purpose of this study, the first primer (Frag I) with product
    length 115 bp, second primer (FragII) with product length 1500 bp, third primer (Frag III)
    with product length 2000 bp that amplify the specific site for the carrier and mild
    patients with G6PD ,while no band was amplified for the chronic patients with G6PD.
    After comparing cases and with NCBI we found that the percentage insertion was 30%
    and substituted was 70%.