A total 200 tumor samples were collected from patients suffering from colorectal CRC at Al-Amal hospital. Their ages ranged from 40 –
70 years old and distributed according to gender as 130 male and 70 female. We found that CRC was more common in male than female,
and more frequent in elderly persons especially in age group of 61 – 70 years old. From 12 specifically designed primers that were able
to amplify KRAS gene in those patients’ blood samples, only 2 gave a specific band when tumor sample was used. Molecular analysis of
the sequence obtained from those two primers showed the presence of 83 missense mutations in both of them, 20 in the first sequence
were associated with pathogenic effect on KRAS gene, 3 frame shift, and 3 insertions, while in the second sequence obtained from primer
PK2
4
frame
shift

mutations, and 4 insertions were identified. The impact finding is that in both sequences, open reading frames
(ORFs) were developed that affected cell proliferation dramatically and produced truncated functionless proteins causing KRAS to cease
control over cell cycle.