ا.م.د. رحاب صبحي رمضان
  • Molecular Analysis of KRAS Mutation Associated with Colorectal Cancer in Iraqi Patients
  • Abstract: The role of KRAS gene was investigated in manifestation of
    colorectal cancer in Iraqi patients. A total 40 blood samples were collected
    during October 2016 to January 2017 from AL-Amal Hospital in Baghdad
    and 20 blood samples from healthy subjects served as control. Blood
    samples were collected from subjects of 40-70 years old for both patients
    and control. The study found that age group 61-70 years old were more
    susceptible to colorectal cancer with ratio of 40% more than younger
    individuals involved in this study with higher frequency in males with
    75.5% than females who show 49.5% (p>0.01) when both compared to
    the same gender. DNA extracted from positive cancer samples and
    control were subjected to specific PCR amplification using 10 specifically
    designed primers for this study to amplify KRAS gene exons. DNA
    sequencing for the resulting amplicons showed the presence of significant
    genetic change that included substitution and insertion, causing 15%
    frame shift and 85% missense changes at positions 5920, consequently
    led to sever disruption in KRAS function.