ا.م.د. رحاب صبحي رمضان
  • Molecular Study of CMV Isolated From Women with Repeated Miscarriage in Relation to Immune Response Molecule TLR2
  •                                          
    This study was constructed to discuss a Molecular Study of Cytomegalovirus
    isolated from women with repeated miscarriage in relation to immune
    response molecule Tall like Receptor2. About (100) blood samples from
    women suffering from infection with Cytomegalovirus were collected from
    infertility clinic of Kamal Al –Sammaraee hospital and (50) samples from
    normal subjects served as control for comparison. Test subjects were divided
    into two age groups: 20-30 years old and 31-40 years old. The women
    distributed as (60) samples of infertile and (40) samples as miscarriage
    women, included molecular analysis by using polymerase chain reaction
    technique (PCR) for amplification two viral genes glycoprotein B (gB) that
    showed molecular weight 72bp and immediate early gene (IE2) that showed
    molecular weight 92bp. The amplification for TLR2 primer region showed
    molecular weight 419bp. After successful amplification of TLR2 gene good
    quality products were selected to be sequenced. The result showed that there
    is no mutation at TLR2 only replacement of C instead of G in wild type.
    PCR amplified for ILT2 primer region showed molecular weight of 285bp. 
    Among ten Iraqi patients, only six give positive result when compared with
    healthy control; the type of mutation is deletion and substitution. The
    percentage of mutation types were deletion mutation (13.3%) and (86.7%)
    for substitution mutation. the effect of mutation was missense mutation
    (72.7%), Silent mutations (15.1%) and deletion mutations (12.2%).