Background: AcneVulgaris (AV) is related to the pilosebaceous follicle. It is considered as adolescent disorder which is characterized by formation of open and closed comedones, papules, pustules, nodules and cysts. Isotretinoin is the only systemic therapy, which has advantages of cure, but causes significant side effects at higher doses.

Objective: The current study was conducted to evaluate serum homocysteine, vitamin B6, vitamin B7, vitamin B12 and folic acid and on sebum composition after treatment with isotretinoin. Methods: This study was conducted on thirty patients with AV. Clinical and laboratory evaluations were conducted before the start of therapy, 6 weeks after, and 12 weeks after treatment. serumhomocysteine, vitamin B6, vitamin B7, vitamin B12, folic acid liver function tests, serum cholesterol and triglyceride, creatinine levels were tested as well assebum cholesterol and sebum squaleneSPSS 20 was used for statistical analysis.

Results: The results of the study showed significant differences (p< 0.05) in homocysteine, vitamin B6, vitamin B7, vitamin B12, folic acid, GGT, ALP, ALT, serum cholesterol, triglyceride, sebum cholesterol and sebum squalene and skin moisture levels, while there were no significant differences in skin temperature, skin skin pH and creatinine.